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Large changes in cytoplasmic biopolymer concentration with osmolality indicate that macromolecular crowding may regulate protein–DNA interactions and growth rate in osmotically stressed Escherichia coli K-12 by Scott Cayley; M. Thomas Record Jr is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Large changes in cytoplasmic biopolymer concentration with osmolality indicate that macromolecular crowding may regulate protein–DNA interactions and growth rate in osmotically stressed Escherichia coli K-12 about?
## Abstract From determination of amounts and concentrations of biopolymers and solutes in the cytoplasm of __Escherichia coli__, we are obtaining information needed to assess the effect of macromolecular crowding on cytoplasmic properties and processes of osmotically stressed bacteria. We observe that growth rate, and the amount of cytoplasmic water decrease and cytoplasmic concentrations of biopolymers and K^+^, increase with increasing osmolality, even for cells grown in the presence of osmoprotectants like glycine betaine. We observe general correlations between the amount of cytoplasmic water, growth rate and cytoplasmic K^+^ concentration in osmotically stressed cells grown both with and without osmoprotectants. To explain these correlations, we propose that crowding increases with increasing growth osmolality, which in turn buffers the binding of proteins to nucleic acids against changes in cytoplasmic K^+^ concentration and (by affecting biopolymer diffusion rates and/or assembly equilibria) is a determinant of growth rate of osmotically stressed cells. Changes in biopolymer concentration and crowding may also explain the increase of the activity coefficient of cytoplasmic
Who reads Large changes in cytoplasmic biopolymer concentration with osmolality indicate that macromolecular crowding may regulate protein–DNA interactions and growth rate in osmotically stressed Escherichia coli K-12?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Scott Cayley; M. Thomas Record Jr
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0952-3499)
- Published
- 2004
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)