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Hybrid Cells Differentiate to Hepatic Lineage Cells and Repair Oxidative Damage by Dan Xu; Feng Wang; Hongyan Gu; Jia Wang; Qinglong Guo; Yanli Zhang; Ziyu Wang is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Hybrid Cells Differentiate to Hepatic Lineage Cells and Repair Oxidative Damage about?
## Abstract Hybrid cells derived from stem cells play an important role in organogenesis, tissue regeneration and cancer formation. However, the fate of hybrid cells and their range of function are poorly understood. Fusing stem cells and somatic cells induces somatic cell reprogramming, and the resulting hybrid cells are embryonic stem cell-like cells. Therefore, we hypothesize that fusion-induced hybrid cells may behave like ES cells in certain microenvironments. In this study, human hepatic cells were induced to apoptosis with H2O2, and then co-cultured with hybrid cells that had been derived from mouse ES cells and human hepatic cells using a transwell. After co-culturing, the degree of apoptosis was evaluated using Annexin-V/PI double-staining analysis, flow cytometry and Western-blot. We observed that H2O2-induced cell apoptosis was inhibited by co-culture. In addition, the activity of injury-related enzymes (GSH-Px, LDH and SOD) and the level of albumin release in the co-culture system trended toward the level of normal undamaged hepatic cells. The stably increased levels of secretion of ALB in the co-culture system also confirmed that co-culture with hybrid cells helped in
Who reads Hybrid Cells Differentiate to Hepatic Lineage Cells and Repair Oxidative Damage?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Dan Xu; Feng Wang; Hongyan Gu; Jia Wang; Qinglong Guo; Yanli Zhang; Ziyu Wang
- Publisher
- SP Versita; Springer (Biomed Central Ltd.); BioMed Central Ltd.; Springer Science and Business Media LLC; Walter de Gruyter GmbH (ISSN 1425-8153)
- Published
- 2010
- Language
- EN
- ISBN
- 9781165801008
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)