Opening book details…
Can I read Identification of Caspase-3 Degradome by Two-dimensional Gel Electrophoresis and Matrix-assisted Laser Desorption/ionization-time of Flight Analysis on EtoBox?
Identification of Caspase-3 Degradome by Two-dimensional Gel Electrophoresis and Matrix-assisted Laser Desorption/ionization-time of Flight Analysis by Ah Young Lee; Byoung Chul Park; Mi Jang; Sayeon Cho; Do Hee Lee; Sang Chul Lee; Pyung Keun Myung; Sung Goo Park is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Identification of Caspase-3 Degradome by Two-dimensional Gel Electrophoresis and Matrix-assisted Laser Desorption/ionization-time of Flight Analysis about?
## Abstract The activation of caspases is a critical event for the execution phase of programmed cell death. Caspases are highly specific in their ability to activate or inhibit many crucial proteins in the cell __via__ site‐specific cleavage. To date, more than 60 proteins have been shown to be substrates of one or more caspases in mammalian cells, and the list is still growing. In this study, to identify human caspase‐3 substrates, we digested lysates obtained from a caspase‐3‐deficient MCF‐7 cell line with purified caspase‐3 and analyzed eliminated or decreased spots by 2‐DE. Proteins degraded by caspase‐3, termed as caspase‐3 degradome, are involved in a variety of cellular functions, such as stress‐responsive proteins, signaling molecules, structural proteins, and unclassified proteins. Interestingly, the cellular level of vinculin, a caspase‐3 substrate, was dramatically reduced during the apoptotic process, where the expression level of caspase‐3 was increased. This degradomic approach could provide a powerful tool in finding physiological substrates of many proteolytic enzymes whose functions remain to be determined.
Who reads Identification of Caspase-3 Degradome by Two-dimensional Gel Electrophoresis and Matrix-assisted Laser Desorption/ionization-time of Flight Analysis?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Ah Young Lee; Byoung Chul Park; Mi Jang; Sayeon Cho; Do Hee Lee; Sang Chul Lee; Pyung Keun Myung; Sung Goo Park
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2004
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)