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Development and application of anin vitro model for screening anti-hepatitis B virus therapeutics by Pietro Lampertico; James S. Malter; Michael A. Gerber is a Medicine article available to read on EtoBox.

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The development of effective anti-hepatitis B virus agents has been hampered by the lack of reliable in uitro systems for the screening of new therapeutics. In an effort to circumvent this problem, we have developed an in uitro system for screening antihepatitis B virus drugs using hepatitis B virus DNA-transfectedHep G2 cells. The cell line designated 2.2.15 produces replicative viral DNA intermediates, mature Dane particles and high levels of viral antigens. Subconfluent 2.2.15 cells were treated with a variety of commonly used anti-hepatitis B virus therapeutics, and their efficacy was determined by analyzing changes in the replicative cellular or extracellular hepatitis B virus DNA content by Southern blotting or slot-blot hybridization. The slot-blot method was sensitive, reproducible and rapid and correlated well with Southern blotting. Analysis of the media for hepatitis B virus DNA was indicative of changes in intracellular, replicative hepatitis B virus DNA, permitting sampling of the media. Therefore 2.2.15 cells may provide a valuable method for identifying and monitoring effective anti-hepatitis B virus therapeutics. Using this system to test various agents, we confirm

Who reads Development and application of anin vitro model for screening anti-hepatitis B virus therapeutics?

Author

Pietro Lampertico; James S. Malter; Michael A. Gerber

Publisher

Ovid Technologies (Wolters Kluwer Health)

Published

1991

Language

EN

Field

Medicine (Health Sciences)