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This lab report summarizes an experiment investigating the light reactions of photosynthesis. The aim was to isolate chloroplasts from spinach leaves and quantify chlorophyll content. The effects of light intensity, ammonia, and DCMU inhibitor on the Hill reaction were then examined. Chloroplasts were isolated via homogenization and centrifugation of spinach leaves. Chlorophyll concentration was determined spectrophotometrically. The Hill reaction was observed by measuring reduction of the DCPIP electron ac

Author

Tiyah Timothy

Language

EN