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Mechanistic Insights Into Actin Force Generation During Vesicle Formation from Cryo-electron Tomography by Daniel Serwas; Matthew Akamatsu; Amir Moayed; Karthik Vegesna; Ritvik Vasan; Jennifer M. Hill; Johannes Schöneberg; Karen M. Davies; Padmini Rangamani; David G. Drubin is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Mechanistic Insights Into Actin Force Generation During Vesicle Formation from Cryo-electron Tomography about?
Actin assembly provides force for a multitude of cellular processes. Compared to actin-assembly-based force production during cell migration, relatively little is understood about how actin assembly generates pulling forces for vesicle formation. Here, cryo-electron tomography identified actin filament number, organization, and orientation during clathrin-mediated endocytosis in human SK-MEL-2 cells, showing that force generation is robust despite variance in network organization. Actin dynamics simulations incorporating a measured branch angle indicate that sufficient force to drive membrane internalization is generated through polymerization and that assembly is triggered from ∼4 founding “mother” filaments, consistent with tomography data. Hip1R actin filament anchoring points are present along the entire endocytic invagination, where simulations show that it is key to pulling force generation, and along the neck, where it targets filament growth and makes internalization more robust. Actin organization described here allowed direct translation of structure to mechanism with broad implications for other actin-driven processes.
Who reads Mechanistic Insights Into Actin Force Generation During Vesicle Formation from Cryo-electron Tomography?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Daniel Serwas; Matthew Akamatsu; Amir Moayed; Karthik Vegesna; Ritvik Vasan; Jennifer M. Hill; Johannes Schöneberg; Karen M. Davies; Padmini Rangamani; David G. Drubin
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)