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N-acetyl Cysteine Prevents Polymethyl Methacrylate Bone Cement Extract-induced Cell Death and Functional Suppression of Rat Primary Osteoblasts by Hideki Aita; Naoki Tsukimura; Masahiro Yamada; Norio Hori; Katsutoshi Kubo; Naoko Sato; Hatsuhiko Maeda; Katsuhiko Kimoto; Takahiro Ogawa is a Engineering article available to read on EtoBox.
What is N-acetyl Cysteine Prevents Polymethyl Methacrylate Bone Cement Extract-induced Cell Death and Functional Suppression of Rat Primary Osteoblasts about?
## Abstract This study examines the cytotoxicity of bone cement extract to osteoblasts and the potential detoxification and restoration of osteoblastic function by an antioxidant amino acid, __N__‐acetyl cysteine (NAC). The osteoblastic cells derived from rat femurs were cultured with extract from polymethyl methacrylate (PMMA)‐based bone cement. The calcein and ethidium homodimer staining of the cells after 24‐h incubation showed that 23.0% of the cells were dead in the culture with bone cement extract, while the addition of 5 m__M__ NAC into the culture reduced the percentage to 4.3%. Annexin V and propidium iodide‐based flow cytometric analysis also revealed that the apoptotic cells present at 15.8% in the culture with bone cement extract was reduced to 2.4% in the culture cotreated with bone cement extract and NAC. Severely suppressed alkaline phosphatase activity and matrix mineralization in the culture with bone cement extract (reduced to 10% and 5%, respectively, compared with the control culture) were restored to a normal level when treated with 5 m__M__ NAC. The bone cement extract‐induced, downregulated expression of osteoblastic genes, such as alkaline phosphatase, colla
Who reads N-acetyl Cysteine Prevents Polymethyl Methacrylate Bone Cement Extract-induced Cell Death and Functional Suppression of Rat Primary Osteoblasts?
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- Author
- Hideki Aita; Naoki Tsukimura; Masahiro Yamada; Norio Hori; Katsutoshi Kubo; Naoko Sato; Hatsuhiko Maeda; Katsuhiko Kimoto; Takahiro Ogawa
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1549-3296)
- Published
- 2009
- Language
- EN
- Field
- Engineering (Physical Sciences)