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Can I read Response Surface Methodology for the Evaluation of Glucose-6-phosphate Dehydrogenase Enrichment Process by Soybean Lecithin Reversed Micelles on EtoBox?
Response Surface Methodology for the Evaluation of Glucose-6-phosphate Dehydrogenase Enrichment Process by Soybean Lecithin Reversed Micelles by Francislene Andréia Hasmann; Daniela de Borba Gurpilhares; Inês Conceição Roberto; Adalberto Pessoa Jr. is a Chemistry article available to read on EtoBox.
What is Response Surface Methodology for the Evaluation of Glucose-6-phosphate Dehydrogenase Enrichment Process by Soybean Lecithin Reversed Micelles about?
Glucose-6-phosphate dehydrogenase (G6PD) present in Saccahromyces cerevisiae is an enzyme of the pentose pathway. An effective enrichment of this intracellular enzyme can be achieved with the reversed micellar methodology. In this work, this methodology was employed with soybean lecithin, a biocompatible surfactant. A factorial design was used to evaluate the influence of pH (A) and extraction runs (B) on the G6PD purification factor. After statistical analysis and process optimization, a mathematical model representing G6PD enrichment was obtained: Y=4.89-0.83A+0.092B+0.27AB-1.37B2 with an enzyme purification factor of about 5.2.
Who reads Response Surface Methodology for the Evaluation of Glucose-6-phosphate Dehydrogenase Enrichment Process by Soybean Lecithin Reversed Micelles?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Francislene Andréia Hasmann; Daniela de Borba Gurpilhares; Inês Conceição Roberto; Adalberto Pessoa Jr.
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-376X)
- Published
- 2007
- Language
- EN
- Field
- Chemistry (Physical Sciences)