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Synthesis and Application of Poly(ethylene Glycol)-co-poly(β-amino Ester) Copolymers for Small Cell Lung Cancer Gene Therapy by Jayoung Kim; Yechan Kang; Stephany Y. Tzeng; Jordan J. Green is a Engineering article available to read on EtoBox.

What is Synthesis and Application of Poly(ethylene Glycol)-co-poly(β-amino Ester) Copolymers for Small Cell Lung Cancer Gene Therapy about?

The design of polymeric nanoparticles for gene therapy requires engineering of polymer structure to overcome multiple barriers, including prolonged colloidal stability during formulation and application. Poly(β-amino ester)s (PBAEs) have been shown effective as polymeric vectors for intracellular DNA delivery, but limited studies have focused on polymer modifications to enhance the stability of PBAE/DNA polyplexes. We developed block copolymers consisting of PBAE oligomer center units and poly(ethylene glycol) (PEG) end units. We fabricated a library of PEG-PBAE polyplexes by blending PEGylated PBAEs of different PEG molecular weights and non-PEGylated PBAEs of different structures at various mass ratios of cationic polymer to anionic DNA. Non-PEGylated PBAE polyplexes aggregated following a 24h incubation in acidic and physiological buffers, presenting a challenge for therapeutic use. In contrast, among 36 PEG-PBAE polyplex formulations evaluated, certain polyplexes maintained a small size under these conditions. These selected polyplexes were further evaluated for transfection in human small cell lung cancer cells (H446) in the presence of serum, and the best formulation transfec

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Author

Jayoung Kim; Yechan Kang; Stephany Y. Tzeng; Jordan J. Green

Published

2016

Language

EN

Field

Engineering (Physical Sciences)