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Spatiotemporal characterization of endothelial cell motility and physical forces during exposure to Borrelia burgdorferi by Marie Muenkel; Raul Aparicio-Yuste; Michal Caspi Tal; Peter Kraiczy; Effie E. Bastounis is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Spatiotemporal characterization of endothelial cell motility and physical forces during exposure to Borrelia burgdorferi about?
## Summary Cell motility and biomechanics are critical in various (patho)physiological processes, including the regulation of vascular barrier integrity, which can be subverted by bacterial pathogens. Here, we present a protocol on how to expose endothelial cells (ECs) to vector-borne __Borrelia burgdorferi__ (__Bb__) and characterize EC kinematics and dynamics during exposure to live or heat-inactivated __Bb__ through traction force and monolayer stress microscopy. Modifications to this protocol may be necessary for studying how different cell types interact with __Bb__ or other microorganisms. For complete details on the use and execution of this protocol, please refer to Yuste et al. (2022).^1^
Who reads Spatiotemporal characterization of endothelial cell motility and physical forces during exposure to Borrelia burgdorferi?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Marie Muenkel; Raul Aparicio-Yuste; Michal Caspi Tal; Peter Kraiczy; Effie E. Bastounis
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)