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Leptin induces increased α2(I) collagen gene expression in cultured rat hepatic stellate cells by Neeraj K. Saxena; George Saliba; Jeffrey J. Floyd; Frank A. Anania is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Leptin is a 16‐kDa hormone with an array of biologic actions. We, and others, have demonstrated that leptin is critical to the development of liver fibrogenesis both in vitro and in the lean littermates of __ob__/__ob__ mice exposed to carbon tetrachloride (CCl~4~). Controversy exists as to whether leptin can act as a direct cytokine in the development of increased collagen expression, and whether __ob__/__ob__ mice are resistant to potential injury from CCl~4~. Here, we provide evidence that strongly suggests that leptin acts to increase nascent production of mRNA for the α2(I) collagen gene based upon ribonuclease protection analysis (RPA). Actinomycin D, but not cyclohexamide, or the pan‐neutralizing antibody to transforming growth factor beta one (TGFβ1), significantly diminished the effect of leptin on total α2(I) collagen mRNA levels. Further evidence that leptin acts directly on HSCs to alter gene expression in liver wounding is demonstrated by enhanced binding of phosphorylated signal transduction and activator of transcription factor 3 (pStat3) to a cis‐inducible element (SIE) oligonucleotide by electrophoretic mobility shift assay (EMSA). This consensus sequen

Who reads Leptin induces increased α2(I) collagen gene expression in cultured rat hepatic stellate cells?

Author

Neeraj K. Saxena; George Saliba; Jeffrey J. Floyd; Frank A. Anania

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)