Can I read Potential of denaturing gradient gel electrophoresis for scanning of ?-thalassemia mutations in India on EtoBox?

Potential of denaturing gradient gel electrophoresis for scanning of ?-thalassemia mutations in India by Gorakshakar, A.C.; Pawar, A.R.; Nadkarni, A.H.; Lu, C.Y.; Mohanty, D.; Krishnamoorthy, R.; Besmond, C.; Colah, R.B. is a Medicine article available to read on EtoBox.

What is Potential of denaturing gradient gel electrophoresis for scanning of ?-thalassemia mutations in India about?

Over the last few years, substantial progress has been made in developing strategies for the detection and characterization of various mutations causing ␤-thalassemia. The Indian population comprises of numerous endogamous caste groups and ␤-thalassemia is seen in almost all of them. Knowledge of the spectrum of ␤-thalassemia mutations in the population is a prerequisite for successful implementation of a prevention programme. Among the different approaches available today, Denaturing Gradient Gel Electrophoresis (DGGE) offers a valid technical approach which is applicable for screening of known mutants and polymorphisms as well as in locating regions of DNA bearing unknown mutations. We analysed 356 unrelated ␤-thalassemia heterozygotes by DGGE and detected 30 anomalous DGGE patterns. Fifteen mutations were characterized after sequencing 25 anomalous patterns. Of these, codon 10(GCC → GCA) is a recently reported novel ␤-thalassemia mutation while -28(A → G) and codon 121(G → T) are being reported for the first time in the Indian population. HbS and HbE also showed two anomalous DGGE patterns each. Framework (FW) linkage studies showed that four mutations were associated with diffe

Who reads Potential of denaturing gradient gel electrophoresis for scanning of ?-thalassemia mutations in India?

Author

Gorakshakar, A.C.; Pawar, A.R.; Nadkarni, A.H.; Lu, C.Y.; Mohanty, D.; Krishnamoorthy, R.; Besmond, C.; Colah, R.B.

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0361-8609)

Published

1999

Language

EN

Field

Medicine (Health Sciences)