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Effect of Stabilizing Additives on the Structure and Hydration of Proteins: a Study Involving Monoclinic Lysozyme by N. T. Saraswathi; R. Sankaranarayanan; M. Vijayan is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Effect of Stabilizing Additives on the Structure and Hydration of Proteins: a Study Involving Monoclinic Lysozyme about?

In pursuance of a long-range programme on the hydration, mobility and action of proteins, the structural basis of the stabilizing effect of sugars and polyols is being investigated. With two crystallographically independent molecules with slightly different packing environments in the crystal, monoclinic lysozyme constitutes an ideal system for exploring the problem. The differences in the structure and hydration of the two molecules provide a framework for examining the changes caused by stabilizing additives. Monoclinic crystals were grown under native conditions and also in the presence of 10% sucrose, 15% trehalose, 10% trehalose, 10% sorbitol and 5% glycerol. The crystal structures were refined at resolutions ranging from 1.8 to 2.1 A. The average B values, and hence the mobility of the structure, are lower in the presence of additives than in the native crystals. However, a comparison of the structures indicates that the effect of the additives on the structure and the hydration shell around the protein molecule is considerably less than that caused by differences in packing. It is also less than that caused by the replacement of NaNO(3) by NaCl as the precipitant in the crys

Who reads Effect of Stabilizing Additives on the Structure and Hydration of Proteins: a Study Involving Monoclinic Lysozyme?

Author

N. T. Saraswathi; R. Sankaranarayanan; M. Vijayan

Publisher

International Union of Crystallography; Blackwell Publishing Inc.; International Union of Crystallography (IUCr) (ISSN 0907-4449)

Published

2002

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)