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HCV recovery from peripheral blood mononuclear cell culture supernatants derived from HCV–HIV co-infected haemophilic patients with undetectable HCV viraemia by P. Baré; I. Massud; L. Belmonte; M. Corti; M. Villafañe; R. Pérez Bianco; M. De Tezanos-pinto; M. M. E. De Bracco; B. Ruibal-Ares is a Medicine article available to read on EtoBox.

What is HCV recovery from peripheral blood mononuclear cell culture supernatants derived from HCV–HIV co-infected haemophilic patients with undetectable HCV viraemia about?

**Summary.** Hepatitis C viraemia, in 38 human immunodeficiency virus positive (HIV+)/hepatitis C virus positive (HCV+) patients, was determined in haemophilic patients during the 4 years since initiation of highly active antiretroviral therapy (HAART). Six of 38 patients had persistently HCV‐negative viraemia for more than 2 years. No correlation between HCV‐negative viraemia and CD4+ T‐cell counts, HIV viral load, age, type or severity of haemophilia could be established. Reduced levels of HIV viral load and the immune reconstitution that follows the initiation of HAART were not enough to explain the disappearance of HCV from plasma. Individuals who cleared plasma HCV had significantly higher CD8+ T‐cell counts (__P__ = 0.0013) (mean ± SE: 1153 ± 117.8cells __μ__L^−1^) than those with HCV‐positive viraemia (819.1 ± 40.72 cells __μ__L^−1^). Because HCV could maintain a low replication level in peripheral blood mononuclear cells (PBMC), we cultured PBMC of five of six patients with undetectable HCV viraemia. We found four of five HCV RNA‐positive cultures. The presence of HCV RNA in our cultures proved that these cells may be an important viral reservoir that could contribute to HC

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Author

P. Baré; I. Massud; L. Belmonte; M. Corti; M. Villafañe; R. Pérez Bianco; M. De Tezanos-pinto; M. M. E. De Bracco; B. Ruibal-Ares

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 1351-8216)

Published

2003

Language

EN

Field

Medicine (Health Sciences)