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This chapter outlines the materials and methods used for synthesizing various nanoparticles (TiO2, Ag, Zn, Au, and their composites) using Cissus quadrangularis leaf extract. It details the preparation of leaf extract, synthesis procedures, surface characterization techniques, and assays for photocatalytic, antimicrobial, antifungal, and anticancer activities. The methodologies include the use of various chemicals and analytical techniques to evaluate the properties and effectiveness of the synthesized nano

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