Can I read Dual Growth Factor-induced Angiogenesis in Vivo Using Hyaluronan Hydrogel Implants on EtoBox?

Dual Growth Factor-induced Angiogenesis in Vivo Using Hyaluronan Hydrogel Implants by Robert A. Peattie; Erin R. Rieke; Erin M. Hewett; Robert J. Fisher; Xiao Zheng Shu; Glenn D. Prestwich is a Engineering article available to read on EtoBox.

What is Dual Growth Factor-induced Angiogenesis in Vivo Using Hyaluronan Hydrogel Implants about?

Crosslinked hyaluronan (HA) hydrogels preloaded with two cytokine growth factors, vascular endothelial growth factor (VEGF) and keratinocyte growth factor (KGF), were employed to elicit new microvessel growth in vivo. As a major glycosaminoglycan (GAG) component of extracellular matrix (ECM), HA is an excellent biopolymeric building block for new biomimetic, biocompatible therapeutic materials. HA hydrogel film samples were surgically implanted in the ear pinnae of mice, and the ears were harvested at 7 or 14 days post-implantation. Histologic analysis showed that each of the groups receiving an implant demonstrated significantly more microvessel density than control ears undergoing surgery but receiving no implant (p<0.001). Treatment groups receiving either co-delivery of both KGF and VEGF, an HA hydrogel lacking a growth factor or HA hydrogels containing a single cytokine were statistically unchanged with time, whereas treatment with KGF alone produced continuing increases in vascularization from day 7 to day 14. Strikingly, presentation of both VEGF and KGF in crosslinked HA generated intact microvessel beds with well-defined borders. In addition, an additive response to co-del

Who reads Dual Growth Factor-induced Angiogenesis in Vivo Using Hyaluronan Hydrogel Implants?

Author

Robert A. Peattie; Erin R. Rieke; Erin M. Hewett; Robert J. Fisher; Xiao Zheng Shu; Glenn D. Prestwich

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0142-9612)

Published

2006

Language

EN

Field

Engineering (Physical Sciences)