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Electrochemical Immunosensor of Platelet-derived Growth Factor with Aptamer-primed Polymerase Amplification by Yong Huang; Xin-Min Nie; Shao-Long Gan; Jian-Hui Jiang; Guo-Li Shen; Ru-Qin Yu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Electrochemical Immunosensor of Platelet-derived Growth Factor with Aptamer-primed Polymerase Amplification about?

A new method for the determination of platelet-derived growth factor BB (PDGF-BB) was developed using an electrochemical immunosensor with an aptamer-primed, long-strand circular detection probe. Rabbit anti-human PDGF-B polyclonal antibody was immobilized on the electrode to serve as the capture antibody. The detection probe was synthesized via polymerase extension along a single-stranded circular plasmid DNA template with a primer headed by the anti-PDGF-B aptamer. In the presence of the analyte, the aptamer-primed circular probe was captured on the electrode via the formation of an antibody/PDGF-BB/aptamer sandwiched complex. The electroactivity indicator methylene blue was adsorbed on the electrode surface via the analyte-sandwiched complex with long-strand circular DNA, thus yielding a strong electrochemical signal for the quantification of PDGF-BB. This strategy allowed electrochemical detection with enormous signal amplification arising from the long-strand localized circular probe. The oxidation peak current of methylene blue in square wave voltammetric measurements showed a linear dependence on the concentration of PDGF-BB in the range from 50 to 500 ng mL(-1), with a dete

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Author

Yong Huang; Xin-Min Nie; Shao-Long Gan; Jian-Hui Jiang; Guo-Li Shen; Ru-Qin Yu

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

2008

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)