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Predictive reconstruction of the mitochondrial iron–sulfur cluster assembly metabolism: I. The role of the protein pair ferredoxin–ferredoxin reductase (Yah1–Arh1) by Rui Alves; Enrique Herrero; Albert Sorribas is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Adrenodoxin reductase homologue (Arh1) and yeast adrenodoxin homologue (Yah1) are essential __Saccharomyces cerevisiae__ mitochondrial proteins involved in heme A biosynthesis and in iron–sulfur cluster (FeSC) assembly. Although the role of Arh1 and Yah1 in heme A biosynthesis is fairly well established, their systemic role on FeSC synthesis is not well understood. Also, while it is thought that the reductase Arh1 provides electrons for the ferredoxin Yah1, two hybrid experiments do not show interaction between the two proteins. In the first part of this article, we use structural bioinformatics methods to evaluate the possibility of interaction between Arh1 and Yah1. Using protein model building and docking algorithms, we predict a complex between Arh1 and Yah1 that is similar to that of their bovine homologues (adrenodoxin reductase–adrenodoxin reductase), suggesting that Arh1 can indeed reduce Yah1. The predicted complex allows us to suggest point mutations to either molecule that could hinder Arh1–Yah1 interaction and test the role of Arh1 as the reductase for Yah1. In the second part of this article, we investigate the physiological role of Arh1–Yah1 on FeSC assemb

Who reads Predictive reconstruction of the mitochondrial iron–sulfur cluster assembly metabolism: I. The role of the protein pair ferredoxin–ferredoxin reductase (Yah1–Arh1)?

Author

Rui Alves; Enrique Herrero; Albert Sorribas

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Liss Inc; Wiley (ISSN 0887-3585)

Published

2004

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)