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Multiple Coupling of Human D5 Dopamine Receptors to Guanine Nucleotide Binding Proteins G~S~ and G~z~ by Anita Sidhu; Kazuhiro Kimura; Misook Uh; Beatrix H. White; Shutish Patel is a Neuroscience article available to read on EtoBox.

What is Multiple Coupling of Human D5 Dopamine Receptors to Guanine Nucleotide Binding Proteins G~S~ and G~z~ about?

Abstract: We have demonstrated previously that D1 dopamine receptors are coupled to both G~s~α and G~o~α. We examine here the coupling between human D5 dopamine receptors and G proteins in transfected rat pituitary GH~4~C~1~ cells. Similar to D1 receptors, cholera toxin treatment of cells reduced, but did not abolish, D5 agonist high‐affinity binding sites, indicating D5 receptors couple to both G~s~α and cholera toxin‐insensitive G proteins. The interaction between D5 receptors and G~s~α was confirmed by immunoprecipitation studies and by the ability of D5 receptors to stimulate adenylyl cyclase. Unlike D1 receptors, D5 receptors did not display any pertussis toxin‐sensitive G‐protein coupling to G~o~α or G~i~α. D5 receptors were also not coupled to G~q~α and were unable to mediate phosphatidylinositol metabolism. Instead, D5 sites appeared to be coupled to an AIF^−^~4~‐sensitive, __N__‐ethylmaleimide‐resistant G protein. Anti‐G~z~α caused immunoprecipitation of 24.2 ± 5.2% of G protein‐associated D5 receptors, indicating coupling between D5 and G~z~α. The coupling to G~z~α was specific for D5 receptors, because similar associations were not detected between D1 receptors and G~z~α

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Author

Anita Sidhu; Kazuhiro Kimura; Misook Uh; Beatrix H. White; Shutish Patel

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)

Published

1998

Language

EN

Field

Neuroscience (Life Sciences)