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Plasmids are circular DNA molecules found in bacteria that serve as important tools for DNA cloning, with engineered plasmids like pBR322 being widely used. pBR322, developed in the 1980s, contains antibiotic resistance genes and is utilized for selecting recombinant plasmids through antibiotic selection and blue-white screening methods. Expression vectors enhance the production of proteins in host cells by including regulatory sequences, though they face limitations such as codon differences and secretion

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