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What is Collagen I Gel Preparation Protocols about?

This document provides protocols for creating collagen I gels from either bovine or rat tail collagen I using DMEM or RPMI cell culture medium. It contains two step-by-step protocols, one for each collagen source. The protocols list the required materials and provide tables with the specific volumes of each ingredient to pipette in order to make gels of different final collagen concentrations. The general procedure is to combine the ingredients on ice, add the collagen last while mixing, then pipette the mi

Author

Omar Hernández

Language

EN