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Drosophila Glia Use a Conserved Cotransporter Mechanism to Regulate Extracellular Volume by William M. Leiserson; Biff Forbush; Haig Keshishian is a Neuroscience article available to read on EtoBox.

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## Abstract The nervous system is protected by blood barriers that use multiple systems to control extracellular solute composition, osmotic pressure, and fluid volume. In the human nervous system, misregulation of the extracellular volume poses serious health threats. Here, we show that the glial cells that form the __Drosophila__ blood–nerve barrier have a conserved molecular mechanism that regulates extracellular volume: the Serine/Threonine kinase Fray, which we previously showed is an ortholog of mammalian PASK/SPAK; and the Na‐K‐Cl cotransporter Ncc69, which we show is an ortholog of human NKCC1. In mammals, PASK/SPAK binds to NKCC1 and regulates its activity. In __Drosophila__, larvae mutant for __Ncc69__ develop a peripheral neuropathy, where fluid accumulates between glia and axons. The accumulation of fluid has no detectable impact on action potential conduction, suggesting that the role of Ncc69 is to maintain volume or osmotic homeostasis. __Drosophila__ Ncc69 has kinetics similar to human NKCC1, and __NKCC1__ can rescue __Ncc69__, suggesting that they function in a conserved physiological mechanism. We show that __fray__ and __Ncc69__ are coexpressed in nerve glia, int

Who reads Drosophila Glia Use a Conserved Cotransporter Mechanism to Regulate Extracellular Volume?

Author

William M. Leiserson; Biff Forbush; Haig Keshishian

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0894-1491)

Published

2010

Language

EN

Field

Neuroscience (Life Sciences)