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Relative transcript quantification by Quantitative PCR: Roughly right or precisely wrong? by Rasmus Skern; Petter Frost; Frank Nilsen is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Background When estimating relative transcript abundances by quantitative real-time PCR (Q-PCR) we found that the results can vary dramatically depending on the method chosen for data analysis. ## Results Analyses of Q-PCR results from a salmon louse starvation experiment show that, even with apparently good raw data, different analytical approaches [1,2] may lead to opposing biological conclusions. ## Conclusion The results emphasise the importance of being cautious when analysing Q-PCR data and indicate that uncritical routine application of an analytical method will eventually result in incorrect conclusions. We do not know the extent of, or have a universal solution to this problem. However, we strongly recommend caution when analysing Q-PCR results e.g. by using two or more analytical approaches to validate conclusions. In our view a common effort should be made to standardise methods for analysis and validation of Q-PCR results.

Who reads Relative transcript quantification by Quantitative PCR: Roughly right or precisely wrong??

Author

Rasmus Skern; Petter Frost; Frank Nilsen

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, [2000]-; Springer Science and Business Media LLC (ISSN 1471-2199)

Published

2005

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)