Opening book details…
Can I read Thrombin Impairs Human Endometrial Endothelial Angiogenesis; Implications for Progestin-only Contraceptive-induced Abnormal Uterine Bleeding on EtoBox?
Thrombin Impairs Human Endometrial Endothelial Angiogenesis; Implications for Progestin-only Contraceptive-induced Abnormal Uterine Bleeding by John P. Shapiro; Ozlem Guzeloglu-Kayisli; Umit A. Kayisli; Nihan Semerci; S. Joseph Huang; Sefa Arlier; Kellie Larsen; Paolo Fadda; Frederick Schatz; Charles J. Lockwood is a Medicine article available to read on EtoBox.
What is Thrombin Impairs Human Endometrial Endothelial Angiogenesis; Implications for Progestin-only Contraceptive-induced Abnormal Uterine Bleeding about?
## Objective: Progestin-only contraceptives induce abnormal uterine bleeding, accompanied by prothrombin leakage from dilated endometrial microvessels and increased thrombin generation by human endometrial stromal cell (hesc)-expressed tissue factor. initial studies of the thrombin-treated hesc secretome identified elevated levels of cleaved chondroitin sulfate proteoglycan 4 (cspg4), impairing pericyte-endothelial interactions. thus, we investigated direct and cspg4-mediated effects of thrombin in eliciting abnormal uterine bleeding by disrupting endometrial angiogenesis. ## Study design: Liquid chromatography/tandem mass spectrometry, enzyme-linked immunosorbent assay (elisa) and quantitative real-time-polymerase chain reaction (pcr) evaluated conditioned medium supernatant and cell lysates from control versus thrombin-treated hescs. pre- and post-depo medroxyprogesterone acetate (dmpa)-administered endometria were immunostained for cspg4. proliferation, apoptosis and tube formation were assessed in human endometrial endothelial cells (heecs) incubated with recombinant human (rh)-cspg4 or thrombin or both. ## Results: Thrombin induced cspg4 protein expression in cultured hescs as
Who reads Thrombin Impairs Human Endometrial Endothelial Angiogenesis; Implications for Progestin-only Contraceptive-induced Abnormal Uterine Bleeding?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- John P. Shapiro; Ozlem Guzeloglu-Kayisli; Umit A. Kayisli; Nihan Semerci; S. Joseph Huang; Sefa Arlier; Kellie Larsen; Paolo Fadda; Frederick Schatz; Charles J. Lockwood
- Publisher
- Elsevier BV
- Published
- 2017
- Language
- EN
- Field
- Medicine (Health Sciences)