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A Water-based Triphenyltetrazolium Chloride Method for the Evaluation of Green Plant Tissue Viability by Chin Ho Lin; Bing Shiunn Chen; Chih Wen Yu; Shih Wen Chiang is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract A water‐based 2,3,5‐triphenyltetrazolium chloride (TTC) partitioning method using __n__‐hexane for the evaluation of green plant tissue viability has been developed. Conventionally, the reduction of TTC to insoluble red‐coloured triphenylformazan (TPF) has been used to detect seed, bud, leaf and cultured cell viability. However, the 95% ethanol used to extract TPF also extracts various pigments, such as chlorophyll, from plant tissues which interfere with the absorption of TPF at 485 nm. This new water‐based method improves upon the current method by eliminating the interfering pigments in the hexane layer, and by minimising the non‐enzymatic oxidation of the sample. When used to evaluate the tissue viability of chill‐stressed waxapple (__Syzygium samarangense__) plants, the refined method had a greater sensitivity than the electrolyte leakage method and thus provided a more precise assessment for the physiological state of various plant tissues. Copyright © 2001 John Wiley & Sons, Ltd.

Who reads A Water-based Triphenyltetrazolium Chloride Method for the Evaluation of Green Plant Tissue Viability?

Author

Chin Ho Lin; Bing Shiunn Chen; Chih Wen Yu; Shih Wen Chiang

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0958-0344)

Published

2001

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)