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Large intergenic non-coding RNA-RoR modulates reprogramming of human induced pluripotent stem cells by Sabine Loewer; Moran N Cabili; Mitchell Guttman; Yuin-Han Loh; Kelly Thomas; In Hyun Park; Manuel Garber; Matthew Curran; Tamer Onder; Suneet Agarwal; Philip D Manos; Sumon Datta; Eric S Lander; Thorsten M Schlaeger; George Q Daley; John L Rinn is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Large intergenic non-coding RNA-RoR modulates reprogramming of human induced pluripotent stem cells about?
The conversion of lineage-committed cells to induced pluripotent stem cells (iPSCs) by reprogramming is accompanied by a global remodeling of the epigenome 1-5 , resulting in altered patterns of gene expression 2,6-9 . Here we characterize the transcriptional reorganization of large intergenic non-coding RNAs (lincRNAs) 10,11 that occurs upon derivation of human iPSCs and identify numerous lincRNAs whose expression is linked to pluripotency. Among these, we defined ten lincRNAs whose expression was elevated in iPSCs compared with embryonic stem cells, suggesting that their activation may promote the emergence of iPSCs. Supporting this, our results indicate that these lincRNAs are direct targets of key pluripotency transcription factors. Using lossof-function and gain-of-function approaches, we found that one such lincRNA (lincRNA-RoR) modulates reprogramming, thus providing a first demonstration for critical functions of lincRNAs in the derivation of pluripotent stem cells. Cellular reprogramming demonstrates the remarkable plasticity of cell fates, as illustrated by the isolation of iPSCs from fibroblasts [6][7][8][9] . Molecular analysis of epigenetic modifications has revealed a
Who reads Large intergenic non-coding RNA-RoR modulates reprogramming of human induced pluripotent stem cells?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Sabine Loewer; Moran N Cabili; Mitchell Guttman; Yuin-Han Loh; Kelly Thomas; In Hyun Park; Manuel Garber; Matthew Curran; Tamer Onder; Suneet Agarwal; Philip D Manos; Sumon Datta; Eric S Lander; Thorsten M Schlaeger; George Q Daley; John L Rinn
- Publisher
- Springer Science and Business Media LLC
- Published
- 2010
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)