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Lysine acetylation plays a role in the allograft-induced stress response of the pearl oyster ( Pinctada fucata martensii ) by Jinzhao Lu,Xiaochen Fang,Haiying Liang,Zhijie Guo,Hexin Zou is a nonfiction available to read on EtoBox.

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Fish and Shellfish Immunology, 130 (2022) 223-232. doi:10.1016/j.fsi.2022.09.018 Lysine acetylation plays a role in the allograft-induced stress response of the pearl oyster (Pinctada fucata martensii) 1 1 Introduction 1 2 Materials and methods 2 2.1 Sample collection and nucleus implantation 2 2.2 Protein extraction and trypsin digestion 3 2.3 Tandem mass tag (TMT) labeling 3 2.4 HPLC (high-pressure liquid chromatography) fractionation and affinity enrichment 3 2.5 LC-MS/MS analysis 3 2.6 Database search 4 2.7 Bioinformatics analysis 4 2.8 Targeted parallel reaction monitoring (PRM) analysis of validation of selected DAPs 4 3 Results 4 3.1 Quality control validation of the MS data 4 3.2 Analysis of the acetylated lysine sites 4 3.3 Differential patterns of acetylation in pearl oysters after allografting 4 3.4 Functional enrichment of the DAPs 5 3.5 PRM data validation 6 4 Discussion 6 5 Conclusion 9 CRediT authorship contribution statement 9 Data availability 9 Acknowledgments 9 References 9 Pinctada fucata martensii,Acetylome,Posttranslational modification,Allografting

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Author

Jinzhao Lu,Xiaochen Fang,Haiying Liang,Zhijie Guo,Hexin Zou

Publisher

Elsevier Ltd

Language

EN

Category

nonfiction