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In situ monitoring of myocardial metabolism by laser fluorimetry: Relevance of a test of local ischemia by Guy Renault; Martine Sinet; Martine Muffat-Joly; Jean Cornillault; Prof. Jean Jacques Pocidalo is a Medicine article available to read on EtoBox.

What is In situ monitoring of myocardial metabolism by laser fluorimetry: Relevance of a test of local ischemia about?

We have developed a hypoxia test by local ischemia, to be performed with a special probe adapted to an in situ NADH laser fluorimeter. Local ischemia is produced by pressurization on the organ surface in an area of approximately 0.2 mm2. In order to assess the method on open-chest rat hearts (ten), we used the following protocol: local ischemia tests (three), global anoxia (100% N2 ventilation), superimposition of local ischemia to global anoxia, and local ischemia tests during the period just prior to death and immediately thereafter. Three different responses were observed: large amplitude of compensated fluorescence (F,) increase, medium amplitude of F, increase, and no F,, increase. These responses were related to the metabolic state prior to the test (States 3, 4, and 5 of Chance's nomenclature). We have thus demonstrated the possibility of very rapidly determining the in situ NADH degree of reduction, without a destructive assay. Such a parameter may be of great relevance in heart surgery, as it might allow detection of potentially harmful situations, thereby enabling early and appropriate treatment.

Who reads In situ monitoring of myocardial metabolism by laser fluorimetry: Relevance of a test of local ischemia?

Author

Guy Renault; Martine Sinet; Martine Muffat-Joly; Jean Cornillault; Prof. Jean Jacques Pocidalo

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0196-8092)

Published

1984

Language

EN

Field

Medicine (Health Sciences)