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Design and Optimization of On-chip Capillary Electrophoresis by Rajiv Bharadwaj; Juan G. Santiago; Bijan Mohammadi is a Engineering article available to read on EtoBox.

What is Design and Optimization of On-chip Capillary Electrophoresis about?

We present a systematic, experimentally validated method of designing electrokinetic injections for on-chip capillary electrophoresis applications. This method can be used to predict point-wise and charge-coupled device (CCD)-imaged electropherograms using estimates of species mobilities, diffusivities and initial sample plug parameters. A simple Taylor dispersion model is used to characterize electrophoretic separations in terms of resolution and signal-to-noise ratio (SNR). Detection convolutions using Gaussian and Boxcar detector response functions are used to relate optimal conditions for resolution and signal as a function of relevant system parameters including electroosmotic mobility, sample injection length, detector length scale, and the length-to-detector. Analytical solutions show a tradeoff between signal-to-noise ratio and resolution with respect to dimensionless injection width and length to the detector. In contrast, there is no tradeoff with respect to the Peclet number as increases in Peclet number favor both SNR and separation solution (R). We validate our model with quantitative epifluorescence visualizations of electrophoretic separation experiments in a simple

Who reads Design and Optimization of On-chip Capillary Electrophoresis?

Author

Rajiv Bharadwaj; Juan G. Santiago; Bijan Mohammadi

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 0173-0835)

Published

2002

Language

EN

Field

Engineering (Physical Sciences)