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Diabetes-induced Changes in the Renal Cortical Proteome Assessed with Two-dimensional Gel Electrophoresis and Mass Spectrometry by Ronald G. Tilton; Sigmund J. Haidacher; Wanda S. LeJeune; Xiaoquan Zhang; Yingxin Zhao; Alexander Kurosky; Allan R. Brasier; Larry Denner is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Diabetes-induced Changes in the Renal Cortical Proteome Assessed with Two-dimensional Gel Electrophoresis and Mass Spectrometry about?
## Abstract To understand the spectrum of proteins affected by diabetes and to characterize molecular functions and biological processes they control, we analyzed the renal cortical proteome of __db/db__ mice using 2‐DE combined with MALDI‐TOF, MALDI‐TOF/TOF, and LC‐MS/MS. This approach yielded 278 high confidence identifications whose expression levels were significantly increased or decreased >two‐fold by diabetes, of which 170 mapped to gene identifiers representing 147 nonredundant proteins. Gene Ontology classification demonstrated that 80% of these proteins modulated physiological functions, 55% involved metabolism, ̃25% involved carboxylic and organic acid metabolism, 14% involved biosynthesis or catabolism, and 12% involved fatty acid metabolism. Predominant molecular functions were catalytic (61%), oxidoreductase (20%), and transferase (17%) activities, and nucleotide and ATP binding (11–15%). Twenty eight percent of the proteins identified as significantly altered by diabetes were mitochondrial proteins. The top‐ranked network described by Ingenuity Pathway Analysis indicated PPARα was the most common node of interaction for the numerous enzymes whose expression levels w
Who reads Diabetes-induced Changes in the Renal Cortical Proteome Assessed with Two-dimensional Gel Electrophoresis and Mass Spectrometry?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Ronald G. Tilton; Sigmund J. Haidacher; Wanda S. LeJeune; Xiaoquan Zhang; Yingxin Zhao; Alexander Kurosky; Allan R. Brasier; Larry Denner
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2007
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)