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Can I read Conditions for a high plating efficiency of free cell suspensions of Haplopappus gracilis (Nutt) gray on EtoBox?
Conditions for a high plating efficiency of free cell suspensions of Haplopappus gracilis (Nutt) gray by P. A. Th. J. Werry; K. M. Stoffelsen is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Conditions for a high plating efficiency of free cell suspensions of Haplopappus gracilis (Nutt) gray about?
Suspensions of Haplopappus gracilis cells, containing about 80% free cells, were obt ained from log-phase cultures by filtration through 3 nylon sieves having decreasing mesh widths from 297, 210 and 88 μm. From the free cell suspensions, 75 to 90% of the cells developed into visible colonies when the plating procedure was divided into two steps: a) plating the cells at high concentration in soft agar on feeder agar; b) replating the resulting aggregations at appropriate concentrations on fresh feeder agar. From the results, it is inferred that, in the replating step, the volume of the inoculum is the deciding factor which influences the resulting plating efficiency.
Who reads Conditions for a high plating efficiency of free cell suspensions of Haplopappus gracilis (Nutt) gray?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- P. A. Th. J. Werry; K. M. Stoffelsen
- Publisher
- Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc.; Springer Nature (ISSN 0040-5752)
- Published
- 1978
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)