Can I read AIP Conference Proceedings [AIP Publishing THE 6TH INTERNATIONAL CONFERENCE ON BIOLOGICAL SCIENCE ICBS 2019: “Biodiversity as a Cornerstone for Embracing Future Humanity” - Yogyakarta, Indonesia (10–11 October 2019)] THE 6TH INTERNATIONAL CONFERENCE ON BIOLOGICAL SCIENCE ICBS 2019: “Biodiversity as a Cornerstone for Embracing Future Humanity” - Development of an agrobacterium-delivered… on EtoBox?

AIP Conference Proceedings [AIP Publishing THE 6TH INTERNATIONAL CONFERENCE ON BIOLOGICAL SCIENCE ICBS 2019: “Biodiversity as a Cornerstone for Embracing Future Humanity” - Yogyakarta, Indonesia (10–11 October 2019)] THE 6TH INTERNATIONAL CONFERENCE ON BIOLOGICAL SCIENCE ICBS 2019: “Biodiversity as a Cornerstone for Embracing Future Humanity” - Development of an agrobacterium-delivered… by Nopitasari, Sri; Setiawati, Yuli; Lawrie, Muhammad Dylan; Purwantoro, Aziz; Widada, Jaka; Sasongko, Aries Bagus; Yoshioka, Yasushi; Matsumoto, Shogo; Ninomiya, Kana; Asano, Yukii; Semiarti, Endang is a Physics and Astronomy article available to read on EtoBox.

What is AIP Conference Proceedings [AIP Publishing THE 6TH INTERNATIONAL CONFERENCE ON BIOLOGICAL SCIENCE ICBS 2019: “Biodiversity as a Cornerstone for Embracing Future Humanity” - Yogyakarta, Indonesia (10–11 October 2019)] THE 6TH INTERNATIONAL CONFERENCE ON BIOLOGICAL SCIENCE ICBS 2019: “Biodiversity as a Cornerstone for Embracing Future Humanity” - Development of an agrobacterium-delivered… about?

CRISPR/Cas9-genome editing system has been developed and started to be used in plants. Phalaenopsis amabilis is one of the popular orchids with beautiful flowers, but flowering time is relatively long, therefore other unique characters need to be created, such as variegated pattern leaves due to chloroplast mutations. VARIEGATA2 (VAR2) gene is the responsible gene for maintaining a chloroplast activity that the mutation will produce a variegated pattern of leaf. The objectives of this research are to get the best method for Agrobacterium-delivered T-DNA containing CRISPR/Cas9 into P.amabilis protocorm and to analyze the mutant phenotype in transformant plants. The genetic transformation was carried out by using A.tumefaciens EHA105 carried T-DNA with UBI::Cas9::U3::VAR2 in pRGEB32, and transformant selected in NP+10 ppm hygromycin medium. T-DNA integration in transformant genomes was confirmed by PCR. The RHS color chart was used for recording plant color of transformant. The results showed transformation efficiency was 1.2%. A color change from green to pale yellowish was detected in the transformants. PCR results showed 545 bp HPT, 402 bp Cas9, 723 bp VAR, and 1200 bp trnL-F were

Who reads AIP Conference Proceedings [AIP Publishing THE 6TH INTERNATIONAL CONFERENCE ON BIOLOGICAL SCIENCE ICBS 2019: “Biodiversity as a Cornerstone for Embracing Future Humanity” - Yogyakarta, Indonesia (10–11 October 2019)] THE 6TH INTERNATIONAL CONFERENCE ON BIOLOGICAL SCIENCE ICBS 2019: “Biodiversity as a Cornerstone for Embracing Future Humanity” - Development of an agrobacterium-delivered…?

Author

Nopitasari, Sri; Setiawati, Yuli; Lawrie, Muhammad Dylan; Purwantoro, Aziz; Widada, Jaka; Sasongko, Aries Bagus; Yoshioka, Yasushi; Matsumoto, Shogo; Ninomiya, Kana; Asano, Yukii; Semiarti, Endang

Publisher

AIP Publishing

Published

2020

Language

EN

Field

Physics and Astronomy (Physical Sciences)