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Can I read Zebrafish as outgroup model to study evolution of scavenger receptor class B type I functions on EtoBox?
Zebrafish as outgroup model to study evolution of scavenger receptor class B type I functions by Robin A.F. Verwilligen; Lindsay Mulder; Pedro M. Araújo; Miguel Carneiro; Jeroen Bussmann; Menno Hoekstra; Miranda Van Eck is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Zebrafish as outgroup model to study evolution of scavenger receptor class B type I functions about?
Background and aims Scavenger receptor class B1 (SCARB1) - also known as the high-density lipoprotein (HDL) receptor - is a multi-ligand scavenger receptor that is primarily expressed in liver and steroidogenic organs. This receptor is known for its function in reverse cholesterol transport (RCT) in mammals and hence disruption leads to a massive increase in HDL cholesterol in these species. The extracellular domain of SCARB1 - which is important for cholesterol handling - is highly conserved across multiple vertebrates, except in zebrafish. Methods: To examine the functional conservation of SCARB1 among vertebrates, two stable scarb1 knockout zebrafish lines, scarb1 715delA (scarb1 −1 nt) and scarb1 715\_716insGG (scarb1 +2 nt), were created using CRISPR-Cas9 technology. Results We demonstrate that, in zebrafish, SCARB1 deficiency leads to disruption of carotenoid-based pigmentation, reduced fertility, and a decreased larvae survival rate, whereas steroidogenesis was unaltered. The observed reduced fertility is driven by defects in female fertility (−50 %, p < 0.001). Importantly, these alterations were independent of changes in free (wild-type 2.4 ± 0.2 μg/μl versus scarb1−/− 2.0
Who reads Zebrafish as outgroup model to study evolution of scavenger receptor class B type I functions?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Robin A.F. Verwilligen; Lindsay Mulder; Pedro M. Araújo; Miguel Carneiro; Jeroen Bussmann; Menno Hoekstra; Miranda Van Eck
- Publisher
- Elsevier BV
- Published
- 2023
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)