Can I read Transient axonal glycoprotein-1 (TAG-1) and laminin-α1 regulate dynamic growth cone behaviors and initial axon direction in vivo on EtoBox?

Transient axonal glycoprotein-1 (TAG-1) and laminin-α1 regulate dynamic growth cone behaviors and initial axon direction in vivo by Marc A Wolman; Vinoth K Sittaramane; Jeffrey J Essner; H Joseph Yost; Anand Chandrasekhar; Mary C Halloran is a Neuroscience article available to read on EtoBox.

What is Transient axonal glycoprotein-1 (TAG-1) and laminin-α1 regulate dynamic growth cone behaviors and initial axon direction in vivo about?

## Background How axon guidance signals regulate growth cone behavior and guidance decisions in the complex __in vivo__ environment of the central nervous system is not well understood. We have taken advantage of the unique features of the zebrafish embryo to visualize dynamic growth cone behaviors and analyze guidance mechanisms of axons emerging from a central brain nucleus __in vivo__. ## Results We investigated axons of the nucleus of the medial longitudinal fascicle (nucMLF), which are the first axons to extend in the zebrafish midbrain. Using __in vivo__ time-lapse imaging, we show that both positive axon-axon interactions and guidance by surrounding tissue control initial nucMLF axon guidance. We further show that two guidance molecules, transient axonal glycoprotein-1 (TAG-1) and laminin-α1, are essential for the initial directional extension of nucMLF axons and their subsequent convergence into a tight fascicle. Fixed tissue analysis shows that TAG-1 knockdown causes errors in nucMLF axon pathfinding similar to those seen in a laminin-α1 mutant. However, __in vivo__ time-lapse imaging reveals that while some defects in dynamic growth cone behavior are similar, there are al

Who reads Transient axonal glycoprotein-1 (TAG-1) and laminin-α1 regulate dynamic growth cone behaviors and initial axon direction in vivo?

Author

Marc A Wolman; Vinoth K Sittaramane; Jeffrey J Essner; H Joseph Yost; Anand Chandrasekhar; Mary C Halloran

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2006-; Springer Science and Business Media LLC (ISSN 1749-8104)

Published

2008

Language

EN

Field

Neuroscience (Life Sciences)