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Noncytotoxic orange and red/green derivatives of DsRed-Express2 for whole-cell labeling by Rita L Strack; Dibyendu Bhattacharyya; Benjamin S Glick; Robert J Keenan is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Noncytotoxic orange and red/green derivatives of DsRed-Express2 for whole-cell labeling about?

## Abstract ## Background Whole-cell labeling is a common application of fluorescent proteins (FPs), but many red and orange FPs exhibit cytotoxicity that limits their use as whole-cell labels. Recently, a tetrameric red FP called DsRed-Express2 was engineered for enhanced solubility and was shown to be noncytotoxic in bacterial and mammalian cells. Our goal was to create derivatives of this protein with different spectral properties. ## Results Building on previous studies of DsRed mutants, we created two DsRed-Express2 derivatives: E2-Orange, an orange FP, and E2-Red/Green, a dual-color FP with both red and green emission. We show that these new FPs retain the low cytotoxicity of DsRed-Express2. In addition, we show that these new FPs are useful as second or third colors for flow cytometry and fluorescence microscopy. ## Conclusion E2-Orange and E2-Red/Green will facilitate the production of healthy, stably fluorescent cell lines and transgenic organisms for multi-color labeling studies.

Who reads Noncytotoxic orange and red/green derivatives of DsRed-Express2 for whole-cell labeling?

Author

Rita L Strack; Dibyendu Bhattacharyya; Benjamin S Glick; Robert J Keenan

Publisher

BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central, 2001-; Springer Science and Business Media LLC (ISSN 1472-6750)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)