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A Novel Sensitive Immunoassay by Nucleic Acid Barcode Dot and Its Application in the Detection of Prostate-specific Antigen by Xiao-Li Kong; Hui Qi; Han-Xin Zhou; Li-Li Ren; Chong-Yan Deng; Fu-Rong Li is a Medicine article available to read on EtoBox.

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## Abstract **Background:** The sensitivity and selectivity of traditional methods limits ultramicro detection of proteins. Bio-barcode amplification detection methods based on nanotechnology enables ultramicro detection of protein. However, bio-barcode amplification detection depends on the oligonucleotides being fixed on a glass chip. It also requires specialized equipment, which limits its application. We introduce a nano-nucleic acid barcode dot detection technology to determine ultramicro concentrations of protein. The method is simple, quick and accurate. **Methods:** Magnetic probe (IgG-M) and dual-labeled gold nanoparticle bio-probe (IgG-Au-DNA) were prepared. Protein was captured using a sandwich assay technique and magnetic separation was used. The DNA barcode was released with dithiothreitol (DTT) and detected directly without the requirement for polymerase chain reaction (PCR). Serum prostate-specific antigen (PSA) from 135 patients was detected with this method and compared with enzyme linked immunosorbent assay (ELISA) and radioimmunoassay (RIA). **Results:** Each IgG-Au-DNA could be covered with 138±47 oligonucleotides and 11±3 antibodies. The IgG-M could bind 118 μg

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Author

Xiao-Li Kong; Hui Qi; Han-Xin Zhou; Li-Li Ren; Chong-Yan Deng; Fu-Rong Li

Publisher

Walter de Gruyter GmbH

Published

2009

Language

EN

Field

Medicine (Health Sciences)