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Membrane and membrane‐associated proteins in Triton X‐114 extracts of ** Mycobacterium bovis ** BCG identified using a combination of gel‐based and gel‐free fractionation strategies by Hiwa Målen; Frode S. Berven; Tina Søfteland; Magnus Øverlie Arntzen; Clive S. D'Santos; Gustavo Antonio De Souza; Harald G. Wiker is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Tuberculosis is an ancient disease that remains a significant global health problem. Because many membrane and membrane‐associated proteins of this pathogen represent potential targets for drugs, diagnostic probes or vaccine components, we have analysed __Mycobacterium bovis__, bacillus Calmette–Guérin (BCG) substrain Moreau, using Triton X‐114 for extraction of lipophilic proteins, followed by identification with LC coupled MS/MS. We identified 351 different proteins in total, and 103 (29%) were predicted as integral membrane proteins with at least one predicted transmembrane region and another 84 (23.9%) proteins had a positive grand average of hydropathicity (GRAVY) value, indicating increased probability for membrane association. Altogether 43 predicted lipoproteins (Lpps) were identified which is close to 50% of the total number of Lpps in the genome. Fifty‐four proteins, including twenty‐four predicted integral membrane proteins and seven predicted Lpps are described for the first time. The proportion of hydrophobic membrane and membrane‐associated proteins shows that Triton X‐114 is a highly efficient method for extraction of membrane proteins from bacteria, with

Who reads Membrane and membrane‐associated proteins in Triton X‐114 extracts of ** Mycobacterium bovis ** BCG identified using a combination of gel‐based and gel‐free fractionation strategies?

Author

Hiwa Målen; Frode S. Berven; Tina Søfteland; Magnus Øverlie Arntzen; Clive S. D'Santos; Gustavo Antonio De Souza; Harald G. Wiker

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)

Published

2008

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)