Opening book details…
Can I read CTCF is a barrier for 2C-like reprogramming on EtoBox?
CTCF is a barrier for 2C-like reprogramming by Olbrich, Teresa (author);Vega-Sendino, Maria (author);Tillo, Desiree (author);Wu, Wei (author);Zolnerowich, Nicholas (author);Pavani, Raphael (author);Tran, Andy D. (author);Domingo, Catherine N. (author);Franco, Mariajose (author);Markiewicz-Potoczny, Marta (author);Pegoraro, Gianluca (author);FitzGerald, Peter C. (author);Kruhlak, Michael J. (author);Lazzerini-Denchi, Eros (author);Nora, Elphege P. (author);Nussenzweig, André (author);Ruiz, Sergio (author) is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is CTCF is a barrier for 2C-like reprogramming about?
Totipotent cells have the ability to generate embryonic and extra-embryonic tissues. Interestingly, a rare population of cells with totipotent-like potential, known as 2 cell (2C)-like cells, has been identified within ESC cultures. They arise from ESC and display similar features to those found in the 2C embryo. However, the molecular determinants of 2C-like conversion have not been completely elucidated. Here, we show that the CCCTC-binding factor (CTCF) is a barrier for 2C-like reprogramming. Indeed, forced conversion to a 2C-like state by the transcription factor DUX is associated with DNA damage at a subset of CTCF binding sites. Depletion of CTCF in ESC efficiently promotes spontaneous and asynchronous conversion to a 2C-like state and is reversible upon restoration of CTCF levels. This phenotypic reprogramming is specific to pluripotent cells as neural progenitor cells do not show 2C-like conversion upon CTCF-depletion. Furthermore, we show that transcriptional activation of the ZSCAN4 cluster is necessary for successful 2C-like reprogramming. In summary, we reveal an unexpected relationship between CTCF and 2C-like reprogramming.
Who reads CTCF is a barrier for 2C-like reprogramming?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Olbrich, Teresa (author);Vega-Sendino, Maria (author);Tillo, Desiree (author);Wu, Wei (author);Zolnerowich, Nicholas (author);Pavani, Raphael (author);Tran, Andy D. (author);Domingo, Catherine N. (author);Franco, Mariajose (author);Markiewicz-Potoczny, Marta (author);Pegoraro, Gianluca (author);FitzGerald, Peter C. (author);Kruhlak, Michael J. (author);Lazzerini-Denchi, Eros (author);Nora, Elphege P. (author);Nussenzweig, André (author);Ruiz, Sergio (author)
- Publisher
- Springer Science and Business Media LLC
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)