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Activation of Excitatory Amino Acid Receptors in the Rat Hippocampal Slice Increases Intracellular Cl^−^ and Cell Volume by Jon R. Inglefield; Rochelle D. Schwartz-Bloom is a Neuroscience article available to read on EtoBox.

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Abstract: The effects of glutamatergic excitotoxins on intracellular Cl^−^ were investigated in the CA1 pyramidal cell layer of the hippocampal slice. Hippocampal slices from rats (14–19 days old) were loaded with 6‐methoxy‐__N__‐ethylquinolinium chloride (MEQ), a Cl^−^‐sensitive fluorescent probe with a fluorescence intensity that correlates inversely with intracellular [Cl^−^]. Slices were exposed for at least 10 min at 26–28°C to __N__‐methyl‐d‐aspartate (NMDA; 100 μ__M__) or α‐amino‐3‐hydroxy‐5‐methylisoxazole‐4‐propionic acid (AMPA; 50 μ__M__). A UV laser scanning confocal microscope was used to measure changes in MEQ fluorescence within area CA1 pyramidal cell soma. Both glutamate receptor agonists produced a rapid decrease in MEQ fluorescence that persisted after washout following a 10‐min exposure. The effects of NMDA and AMPA were prevented by the competitive antagonists 2‐amino‐5‐phosphonopentanoic acid and 6,7‐dinitroquinoxaline‐2,3‐dione, respectively. Neither tetrodotoxin nor picrotoxin prevented the effect of NMDA or AMPA, indicating the lack of involvement of presynaptic mechanisms. The effects of NMDA and AMPA on MEQ fluorescence were dependent on the levels of extr

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Author

Jon R. Inglefield; Rochelle D. Schwartz-Bloom

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)

Published

1998

Language

EN

Field

Neuroscience (Life Sciences)