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This study investigates the cellular growth dynamics and allelopathic activity of coffee cell cultures, specifically Coffea canephora, using a modified sandwich method bioassay. Results indicate that coffee callus exhibits significant growth inhibition on lettuce seedlings, with the strongest allelopathic effects observed during the lag phase of growth. High-performance liquid chromatography analysis identified key allelochemicals, including theobromine and caffeine, suggesting a correlation between growth

Author

keiki.kitamura

Language

EN