Can I read RT-PCR Protocols (Methods in Molecular Biology) on EtoBox?

RT-PCR Protocols (Methods in Molecular Biology) by Joseph O'Connell Joe O'Connell is a nonfiction available to read on EtoBox.

What is RT-PCR Protocols (Methods in Molecular Biology) about?

RT-PCR, the most widely used technique in biomedical research for analyzing gene expression, has proven a significant boon to the molecular investigation of disease pathogenesis. In RT-PCR Protocols, Joe O'Connell has assembled a panel of highly regarded molecular biologists and clinical researchers to describe in detail their most novel, useful, and interesting RT-PCR applications. Here the newcomer will find readily reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For the expert seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering. Each protocol emphasizes the technical steps critical for experimental success and includes tips on avoiding potential pitfalls, notes explaining the method's scientific basis and its possible sources of error, and comments on how it

Who reads RT-PCR Protocols (Methods in Molecular Biology)?

Author

Joseph O'Connell Joe O'Connell

Publisher

Totowa, N.J.: Humana Press

Published

2002

Language

EN

ISBN

9781592592838

Category

nonfiction

Subjects

Science, Chemistry, Medical