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Design of New Enzyme Stabilizers Inspired by Glycosides of Hyperthermophilic Microorganisms by Tiago Q. Faria; Ana Mingote; Filipa Siopa; Rita Ventura; Christopher Maycock; Helena Santos is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Design of New Enzyme Stabilizers Inspired by Glycosides of Hyperthermophilic Microorganisms about?
In response to stressful conditions like supra-optimal salinity in the growth medium or temperature, many microorganisms accumulate low-molecular-mass organic compounds known as compatible solutes. In contrast with mesophiles that accumulate neutral or zwitterionic compounds, the solutes of hyperthermophiles are typically negatively charged. (2R)-2-(a-D-Mannopyranosyl)glycerate (herein abbreviated as mannosylglycerate) is one of the most widespread solutes among thermophilic and hyperthermophilic prokaryotes. In this work, several molecules chemically related to mannosylglycerate were synthesized, namely The effectiveness of the newly synthesized compounds for the protection of model enzymes against heat-induced denaturation, aggregation and inactivation was evaluated, using differential scanning calorimetry, light scattering and measurements of residual activity. For comparison, the protection induced by natural compatible solutes, either neutral (e.g., trehalose, glycerol, ectoine) or negatively charged (di-myo-inositol-1,3 0 -phosphate and diglycerol phosphate), was assessed. Phosphate, sulfate, acetate and KCl were also included in the assays to rank the solutes and new compoun
Who reads Design of New Enzyme Stabilizers Inspired by Glycosides of Hyperthermophilic Microorganisms?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Tiago Q. Faria; Ana Mingote; Filipa Siopa; Rita Ventura; Christopher Maycock; Helena Santos
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0008-6215)
- Published
- 2008
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)