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Oocyte Arrested at Metaphase II Stage were Derived from Human Pluripotent Stem Cells in vitro by Xiaoli Yu; Ning Wang; Xiang Wang; Hehe Ren; Yanping Zhang; Yingxin Zhang; Yikai Qiu; Hongyan Wang; Guoping Wang; Xiuying Pei; Ping Chen; Yahui Ren; Chunfang Ha; Li Wang; Huayan Wang is a Medicine article available to read on EtoBox.
What is Oocyte Arrested at Metaphase II Stage were Derived from Human Pluripotent Stem Cells in vitro about?
Initiation of meiosis is the most difficult aspect of inducing competent oocytes differentiation from human stem cells __in vitro__. Human induced pluripotent stem cells (hiPSCs) and embryonic stem cells (hESCs) were cultured with follicle fluid, cytokines and small molecule to induced oocyte-like cells (OLCs) formation through a three-step induction procedure. Expression of surface markers and differentiation potential of germ cells were analyzed __in vitro__ by flow cytometry, gene expression, immunocytochemistry, western blotting and RNA Sequencing. To induce the differentiation of hiPSCs into OLCs, cells were firstly cultured with a primordial germ cell medium for 10 days. The cells exhibited similar morphological features to primordial germ cells (PGCs), high expressing of germ cell markers and primordial follicle development associated genes. The induced PGCs were then cultured with the primordial follicle-like cell medium for 5 days to form the induced follicle-like structures (iFLs), which retained both primordial oocytes-like cells and granulosa-like cells. In the third step, the detached iFLs were harvested and transferred to the OLC-medium for additional 10 days. The cul
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It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Xiaoli Yu; Ning Wang; Xiang Wang; Hehe Ren; Yanping Zhang; Yingxin Zhang; Yikai Qiu; Hongyan Wang; Guoping Wang; Xiuying Pei; Ping Chen; Yahui Ren; Chunfang Ha; Li Wang; Huayan Wang
- Publisher
- Springer Science and Business Media LLC
- Published
- 2023
- Language
- EN
- Field
- Medicine (Health Sciences)