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Gene regulation in intertypic heterokaryons of Solanum tuberosum and Nicotiana tabacum tissue protoplasts by W. J. P. van Kesteren; E. W. Bijmolt; M. J. Tempelaar is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## ABSTRACT Activities of the ß‐glucuronidase (GUS) reporter enzyme were evaluated in transgenic plants, protoplasts, and intertypic heterokaryons of __Solanum tuberosum__ and __Nicotiana tabacum__. With GUS under control of the promoter of the cauliflower‐mosaicvirus 35S RNA gene (CaMV), activities of the enzyme were nearly evenly distributed over the tissues of plants grown __in vitro__. Activities in microtubers of potato plants with the reporterenzyme under control of the promoter of granule‐bound starch synthase (GBSS) from __S. tuberosum__ were higher than in leaves. The CaMV‐GUS construct present in leaf protoplasts showed an increased expression in biochemical and cytochemical assays after fusion with wildtype‐tuber protoplasts. Such an increase was not observed in the case of the GBSS‐GUS constructs. It was concluded that nuclei in plant heterokaryons are being influenced by the fusion partner, but that repressive, __trans__‐acting regulation factors in leaf protoplasts possibly prevent an increase of the expression of the chimeric GBSS‐gene in heterokaryons.

Who reads Gene regulation in intertypic heterokaryons of Solanum tuberosum and Nicotiana tabacum tissue protoplasts?

Author

W. J. P. van Kesteren; E. W. Bijmolt; M. J. Tempelaar

Publisher

Elsevier Science; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 1065-6995)

Published

1994

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)