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TIME-seq reduces time and cost of DNA methylation measurement for epigenetic clock construction by Griffin, Patrick T. (author);Kane, Alice E. (author);Trapp, Alexandre (author);Li, Jien (author);Arnold, Matthew (author);Poganik, Jesse R. (author);Conway, Ryan J. (author);McNamara, Maeve S. (author);Meer, Margarita V. (author);Hoffman, Noah (author);Amorim, João A. (author);Tian, Xiao (author);MacArthur, Michael R. (author);Mitchell, Sarah J. (author);Mueller, Amber L. (author);Carmody, Colleen (author);Vera, Daniel L. (author);Kerepesi, Csaba (author);Ying, Kejun (author);Noren Hooten, Nicole (author);Mitchell, James R. (author);Evans, Michele K. (author);Gladyshev, Vadim N. (author);Sinclair, David A. (author) is a Medicine article available to read on EtoBox.

What is TIME-seq reduces time and cost of DNA methylation measurement for epigenetic clock construction about?

Epigenetic 'clocks' based on DNA methylation have emerged as the most robust and widely used aging biomarkers, but conventional methods for applying them are expensive and laborious. Here we develop tagmentationbased indexing for methylation sequencing (TIME-seq), a highly multiplexed and scalable method for low-cost epigenetic clocks. Using TIME-seq, we applied multi-tissue and tissue-specific epigenetic clocks in over 1,800 mouse DNA samples from eight tissue and cell types. We show that TIMEseq clocks are accurate and robust, enriched for polycomb repressive complex 2-regulated loci, and benchmark favorably against conventional methods despite being up to 100-fold less expensive. Using dietary treatments and gene therapy, we find that TIME-seq clocks reflect diverse interventions in multiple tissues. Finally, we develop an economical human blood clock (R > 0.96, median error = 3.39 years) in 1 ,0 56 d em ographically representative individuals. These methods will enable more efficient epigenetic clock measurement in larger-scale human and animal studies. Aging is difficult to study, in part because it is difficult to quantify 1 . In recent years, researchers attempted to address

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Author

Griffin, Patrick T. (author);Kane, Alice E. (author);Trapp, Alexandre (author);Li, Jien (author);Arnold, Matthew (author);Poganik, Jesse R. (author);Conway, Ryan J. (author);McNamara, Maeve S. (author);Meer, Margarita V. (author);Hoffman, Noah (author);Amorim, João A. (author);Tian, Xiao (author);MacArthur, Michael R. (author);Mitchell, Sarah J. (author);Mueller, Amber L. (author);Carmody, Colleen (author);Vera, Daniel L. (author);Kerepesi, Csaba (author);Ying, Kejun (author);Noren Hooten, Nicole (author);Mitchell, James R. (author);Evans, Michele K. (author);Gladyshev, Vadim N. (author);Sinclair, David A. (author)

Publisher

Springer Science and Business Media LLC

Published

2024

Language

EN

Field

Medicine (Life Sciences)