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Can I read Monitoring the Production of Biosynthetic Human Growth Hormone by Micro Enzyme-linked Immunosorbent Assay on EtoBox?
Monitoring the Production of Biosynthetic Human Growth Hormone by Micro Enzyme-linked Immunosorbent Assay by Bo Dinesen; Henrik Dalbøge Andersen is a Chemistry article available to read on EtoBox.
What is Monitoring the Production of Biosynthetic Human Growth Hormone by Micro Enzyme-linked Immunosorbent Assay about?
A micro enzyme-linked immunosorbent assay (e.1.i.s.a.) has been developed for monitoring the production of human growth hormone (hGH) in E. coli. The method is unusually flexible, as it is possible to modify its conditions to give a sensitive (detection limit 4 ng 1-l) or a fast (6 h) version. The assay is reproducible; the between-assay relative standard deviation is 6%. The effects of temperature, incubation time and the concentrations of protein and the most important reagents (the solid-phase antibody and the labelled antibody) are described. The utility of the method is demonstrated in experiments optimizing the conditions for production of hGH in E. coli.
Who reads Monitoring the Production of Biosynthetic Human Growth Hormone by Micro Enzyme-linked Immunosorbent Assay?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Bo Dinesen; Henrik Dalbøge Andersen
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0003-2670)
- Published
- 1984
- Language
- EN
- Field
- Chemistry (Physical Sciences)