Can I read Determination of Ca2+/Calmodulin-Stimulated Phosphodiesterase Activity in Intact Cells on EtoBox?

Determination of Ca2+/Calmodulin-Stimulated Phosphodiesterase Activity in Intact Cells by Lugnier, Claire is a scholarly article available to read on EtoBox.

What is Determination of Ca2+/Calmodulin-Stimulated Phosphodiesterase Activity in Intact Cells about?

The multigenic family of cyclic nucleotide phosphodiesterases (PDEs) is now known to offer many new therapeutic possibilities through their influence over intracellular signaling, though their precise cell mechanisms to modulating that process remain uncertain. In Phosphodiesterase Methods and Protocols, a panel of research leaders in the PDE field describes new concepts and techniques for investigating the role of PDEs in orchestrating normal and pathophysiological responses. Presented in step-by-step detail, these readily reproducible methods allow the measurement of cyclic nucleotide variations in living cells, as well as their visualization in a spatiotemporal manner, the localization and characterization of their activities in tissues and living cells, and the assessment of targeted PDEs in creating specific tools and drugs. Additional chapters discuss the generation of PDE4 knockout mice to demonstrate not only the potential role of targeted PDEs, but also their use in further studies of the central role of PDE regulation in intracellular signaling control. These techniques offer clinicians a way to find new therapies for numerous pathologies whose molecular origins are unkno

Author

Lugnier, Claire

Publisher

Humana Press : Imprint : Humana

Published

2005

Language

EN

ISBN

9781592598397