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Can I read Molecular superglue-mediated higher-order assembly of TRAIL variants with superior apoptosis induction and antitumor activity on EtoBox?
Molecular superglue-mediated higher-order assembly of TRAIL variants with superior apoptosis induction and antitumor activity by Hao Yang; Heng Li; Fen Yang; Ze Tao; Qiuxiao Shi; Tianshan She; Yanru Feng; Zhao Li; Jie Chen; Yi Zhong; Tao Su; Wengjuan Zeng; Yong Zhang; Shisheng Wang; Lan Li; Tingting Long; Dan Long; Jingqiu Cheng; Hong Zhu; Xiaofeng Lu is a Engineering article available to read on EtoBox.
What is Molecular superglue-mediated higher-order assembly of TRAIL variants with superior apoptosis induction and antitumor activity about?
Prompting higher-order death receptor (DR) clustering by increasing the valency of DR agonist is efficient to induce apoptosis of tumor cells. As an attractive DR agonist with superior biosafety, the trimeric tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) exerts limited antitumor effect in patients, which is predominantly attributed to its low DR clustering ability and short serum half-life. Previous antibody scaffolds-based engineering strategies to increase the valency and/or prolong the serum half-life of TRAIL improve apoptosis induction, however, often produce large proteins with poor tumor penetration. Covalent protein ligation mediated by small molecular superglues such as SpyTag/SpyCatcher might be a novel strategy to assemble higher-order TRAIL variants. Upon fusion to TRAIL promotor, SpyTag/SpyCatcher molecular superglue preferentially ligated two trimeric TRAIL to produce a hexameric TRAIL variant, HexaTR, exhibiting a significantly increased apoptosis induction. In addition, an albumin-binding HexaTR, ABD-HexaTR, with a prolonged serum half-life by binding to endogenous albumin was also produced using the same strategy. Compared to the trimeric TRAIL, t
Who reads Molecular superglue-mediated higher-order assembly of TRAIL variants with superior apoptosis induction and antitumor activity?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Hao Yang; Heng Li; Fen Yang; Ze Tao; Qiuxiao Shi; Tianshan She; Yanru Feng; Zhao Li; Jie Chen; Yi Zhong; Tao Su; Wengjuan Zeng; Yong Zhang; Shisheng Wang; Lan Li; Tingting Long; Dan Long; Jingqiu Cheng; Hong Zhu; Xiaofeng Lu
- Publisher
- Elsevier BV
- Published
- 2023
- Language
- EN
- Field
- Engineering (Physical Sciences)