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Differences in the Lectin-binding Patterns of the Periportal and Perivenous Endothelial Domains in the Liver Sinusoids by Emilio Barberá-Guillem; Marian Rocha; Antonia Alvarez; Fernando Vidal-Vanaclocha is a Medicine article available to read on EtoBox.

What is Differences in the Lectin-binding Patterns of the Periportal and Perivenous Endothelial Domains in the Liver Sinusoids about?

We have studied the distribution patterns of carbohydrate terminals on the endothelial surface of the mouse liver microvasculature. For this purpose, a wide battery of FITC lectins specific to glucose, mannose, galactose, fucose, N-acetyl-neuraminic acid, N-acetylgalactosamine and N-acetyl-glucosamine residues were incubated on liver cryostat sections or intraportally perfused under physiological conditions. All the resulting hepatic sections were examined under fluorescent microscopy and confocal laser scanning microscopy. With the exception of N-acetylgalactosamine-and fucose-binding lectins, all the per- fused lectins specifically bound to the microvascular wall as confirmed by blocking methods using their correeponding sugars. A wide range of binding was, however, observed among the lectins, and the latter were classified into four groups according to their affinities for the different segments of the hepatic microvasculature: (a) equal affinity for all segments (concanavalin A); (b) different affinities depending on acinar zone (wheat germ agglutinin, Ricinus communis toxin, phytohemagglutinin E, Erythrinu cristczgulli agglutinin and Pisum sutiuun agglutinin); (c) preferential

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Author

Emilio Barberá-Guillem; Marian Rocha; Antonia Alvarez; Fernando Vidal-Vanaclocha

Publisher

Ovid Technologies (Wolters Kluwer Health)

Published

1991

Language

EN

Field

Medicine (Health Sciences)