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Capillary zone electrophoresis of sub-μm-sized particles in electrolyte solutions of various ionic strengths: Size-dependent electrophoretic migration and separation efficiency by Sergey P. Radko; Miroslava Stastna; Andreas Chrambach is a Engineering article available to read on EtoBox.

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To gain insight into the mechanisms of size-dependent separation of microparticles in capillary zone electrophoresis (CZE), sulfated polystyrene latex microspheres of 139, 189, 268, and 381 nm radius were subjected to CZE in Tris-borate buffers of various ionic strengths ranging from 0.0003 to 0.005, at electric field strengths of 100±500 V cm ±1 . Size-dependent electrophoretic migration of polystyrene particles in CZE was shown to be an explicit function of kR, where k ±1 and r are the thickness of electric double layer (which can be derived from the ionic strength of the buffer) and particle radius, respectively. Particle mobility depends on kR in a manner consistent with that expected from the Overbeek-Booth electrokinetic theory, though a charged hairy layer on the surface of polystyrene latex particles complicates the quantitative prediction and optimization of size-dependent separation of such particles in CZE. However, the Overbeek-Booth theory remains a useful general guide for size-dependent separation of microparticles in CZE. In accordance with it, it could be shown that, for a given pair of polystyrene particles of different sizes, there exists an ionic strength which

Who reads Capillary zone electrophoresis of sub-μm-sized particles in electrolyte solutions of various ionic strengths: Size-dependent electrophoretic migration and separation efficiency?

Author

Sergey P. Radko; Miroslava Stastna; Andreas Chrambach

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 0173-0835)

Published

2000

Language

EN

Field

Engineering (Physical Sciences)